Tg(Cela1-cre/ERT)1Lgdn
Transgene Detail

Summary

• Symbol: Tg(Cela1-cre/ERT)1Lgdn
• Name: transgene insertion 1, Craig Logsdon
• MGI ID: MGI:3848026
• Synonyms: BAC-Ela-CreErT, Ela-Cre-ERT2
• Transgene: Tg(Cela1-cre/ERT)1Lgdn Location: unknown
• Alliance: Tg(Cela1-cre/ERT)1Lgdn page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Cela1-cre/ERT)1Lgdn expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Cela1 (MGI:95314)

• Mutation details: A mouse BAC (clone RP23-359O19, size 222 kb) carrying the intact elastase I (Cela1) gene was used. The BAC was modified to express CreERT. For recombination-mediated targeting, arms homologous to the sequence of elastase I gene between the last exon and the polyA signal (in lower case) were added to either side of the IRES-Cre-ERT-frt-Kan-frt cassette by PCR. Using homologous recombination, the IRES-Cre-ERT-frt-Kan-frt cassette was inserted into the elastase I gene. The Kan selection marker was then removed by flp-mediated recombination. two BAC-Cela1-CreERT founders were identified by genotyping. Q-PCR based analysis indicated the presence of 2-3 copies of the transgene in BAC-Cela1-CreERT founders. (J:148748)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cela1 (mouse) Summary of all recombinase alleles driven by Cela1. Comparative matrix view of recombinase activities Alliance mouse Cela1 gene page

Tumor Data

List all tumor models in MMHCdb carrying Tg(Cela1-cre/ERT)1Lgdn

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:148748 Ji B, et al., Robust acinar cell transgene expression of CreErT via BAC recombineering. Genesis. 2008 Aug;46(8):390-5
• All: 27 reference(s)