Glis3^tm1Amj
Targeted Allele Detail

Summary

• Symbol: Glis3^tm1Amj
• Name: GLIS family zinc finger 3; targeted mutation 1, Anton M Jetten
• MGI ID: MGI:3848053
• Synonyms: Glis3^deltaZF5, Glis3^zf
• Gene: Glis3 Location: Chr19:28236251-28657477 bp, - strand Genetic Position: Chr19, 22.88 cM
• Alliance: Glis3^tm1Amj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148994
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4, which contains the sequence coding for zinc finger 5, and parts of intron 3 and 4 were replaced with a neo cassette. Deletion of zinc finger 5 (ZF5) is predicted to disrupt DNA binding ablity and transcriptional activity of the gene. The inability of the protein produced from this allele to activate transcription of a luciferase reporter with a Glis-binding site was confirmed by cellular assay. (J:148994)

Disease models

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Glis3 Mutation: 40 strains or lines available

References

• Original: J:148994 Kang HS, et al., Glis3 is associated with primary cilia and Wwtr1/TAZ and implicated in polycystic kidney disease. Mol Cell Biol. 2009 May;29(10):2556-69
• All: 5 reference(s)