Jag1^tm1.1Loo
Targeted Allele Detail

Summary

• Symbol: Jag1^tm1.1Loo
• Name: jagged 1; targeted mutation 1.1, Kathleen M Loomes
• MGI ID: MGI:3848155
• Synonyms: Jag1^loxP
• Gene: Jag1 Location: Chr2:136923371-136958440 bp, - strand
• Alliance: Jag1^tm1.1Loo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149131
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neomycin cassette was placed upstream of exon 4 with an additional loxP site placed downstream of exon 5. The neomycin cassette was removed by transient expression of cre recombinase leaving exons 4 and 5 floxed. Exon 4 encodes the DSL region, which is conserved across species and is known to be crucial for ligand-receptor interactions. Deletion of exon 4 and exon 5 by cre recombinase was predicted to result in a frameshift mutation and premature truncation of the protein after 192 amino acids. (J:149131)

Expression

In Mice Carrying this Mutation:	114 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jag1 Mutation: 78 strains or lines available

References

• Original: J:149131 Loomes KM, et al., Bile duct proliferation in liver-specific Jag1 conditional knockout mice: effects of gene dosage. Hepatology. 2007 Feb;45(2):323-30
• All: 7 reference(s)