Itgb1^tm5.1Ref
Targeted Allele Detail

Summary

• Symbol: Itgb1^tm5.1Ref
• Name: integrin beta 1 (fibronectin receptor beta); targeted mutation 5.1, Reinhard Fassler
• MGI ID: MGI:3848527
• Synonyms: hpmKI^lox
• Gene: Itgb1 Location: Chr8:129412135-129459681 bp, + strand Genetic Position: Chr8, 76.09 cM, cytoband E2
• Alliance: Itgb1^tm5.1Ref page

Hair and skin phenotypes of various keratinocyte conditional Itgb1 alleles

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148885
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutation: Insertion Mutation details: A 141 bp cDNA fragment of the mouse Itgb1 gene encoding for the entire cytoplasmic tail including the stop codon was inserted in frame into exon 15 and a floxed neo-tk cassette was inserted into intron 15 via homologous recombination. Cre mediated recombination then removed the neo-tk cassette leaving a single loxP site in intron 15. Analysis indicated that the mutant mRNA undergoes nonsense mediated decay. (J:148885)

Generation of the Itgb1^tm5.1Ref allele

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Itgb1 Mutation: 60 strains or lines available

References

• Original: J:148885 Piwko-Czuchra A, et al., Beta1 integrin-mediated adhesion signalling is essential for epidermal progenitor cell expansion. PLoS One. 2009;4(5):e5488
• All: 3 reference(s)