Tg(Mt1-LDLR)93-4Reh
Transgene Detail

Summary

• Symbol: Tg(Mt1-LDLR)93-4Reh
• Name: transgene insertion 93-4, Robert E Hammer
• MGI ID: MGI:3848828
• Synonyms: hLDLRTg, mMT-I-hLDLR-hGH
• Transgene: Tg(Mt1-LDLR)93-4Reh Location: unknown
• Alliance: Tg(Mt1-LDLR)93-4Reh page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Mt1-LDLR)93-4Reh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  LDLR (3949)

  Ldlr	(MGI:96765)

• Mutation details: Transgenic mice were created in the laboratory of Dr. Robert E Hammer (University of Texas Southwestern Medical Center). The MMT-I-hLDLR transgene was designed with a 1.7 kb fragment of mouse metallothionein 1 gene (Mt1) promoter, a 2.6 kb cDNA of the human low density lipoprotein receptor (hLDLR) complete coding region (and 27 basepairs of 5' UTR), and the transcription termination signal from the human growth hormone gene. This transgene was microinjected into the male pronuclei of fertilized (C57/SJL)F2 hybrid mouse eggs, Founder mouse 93-4, with high hLDLR expression in the liver, was bred to establish the colony. These mice have 3 copies of the transgene inserted at a single site and express human LDLR mRNA. (J:127519)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:127519 Hofmann SL, et al., Overexpression of low density lipoprotein (LDL) receptor eliminates LDL from plasma in transgenic mice. Science. 1988 Mar 11;239(4845):1277-81
• All: 2 reference(s)