Dlg4^tm2.1Grnt
Targeted Allele Detail

Summary

• Symbol: Dlg4^tm2.1Grnt
• Name: discs large MAGUK scaffold protein 4; targeted mutation 2.1, Seth G N Grant
• MGI ID: MGI:3848928
• Synonyms: PSD-95^GK
• Gene: Dlg4 Location: Chr11:69908029-69938107 bp, + strand Genetic Position: Chr11, 42.96 cM, cytoband B4
• Alliance: Dlg4^tm2.1Grnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89778
• Parent Cell Line: E14TG2aIV (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A STOP codon and coding sequence for haemaglutinin (HA) epitope tag were inserted in frame before the start of the GK domain at amino acid 500. In addition, a floxed neo cassette was inserted downstream to replace sequence and this cassette was subsequently excised by Cre leaving a single loxP site after the STOP codon. Western blot and immunoprecipitation analysis confirmed the absence of protein in hippocampal extracts from homozygous mice. RT-PCR analysis indicated that a very low level of mRNA expression (less than 5% of wild-type) was present in hippocampal extracts from homozygous mice. (J:149508)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dlg4 Mutation: 46 strains or lines available

References

• Original: J:89778 Yao WD, et al., Identification of PSD-95 as a regulator of dopamine-mediated synaptic and behavioral plasticity. Neuron. 2004 Feb 19;41(4):625-38
• All: 11 reference(s)