Lmx1b^{tm3(cre/ERT2)Rjo}
Targeted Allele Detail

Summary

• Symbol: Lmx1b^{tm3(cre/ERT2)Rjo}
• Name: LIM homeobox transcription factor 1 beta; targeted mutation 3, Randy L Johnson
• MGI ID: MGI:3849051
• Synonyms: Lmx1b^N246K-Cre, Lmx1b^N246K-IREScreER^T2, Lmx1b^PM-IREScreER^T2
• Gene: Lmx1b Location: Chr2:33450977-33530620 bp, - strand Genetic Position: Chr2, 22.48 cM
• Alliance: Lmx1b^{tm3(cre/ERT2)Rjo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149241
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutations: Insertion, Single point mutation
• Lmx1b^{tm3(cre/ERT2)Rjo} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lmx1b (MGI:1100513)

• Mutation details: A C->A transition (changing the asparagine N, at position 246 to lysine, K) was introduced into exon 5 to produce a null allele of Lmx1b. Subsequently, an IREScreERT2 cassette, followed by a loxP-flanked PGK-neo, was inserted into the 3'UTR of the locus by homologous recombination in PC3 ES cells. Cre mediated recombination then removed the PGK-neo cassette upon transmission from male chimeric mice. The protamine-cre transgene contributed by the PC3 cells was separated from the knock-in allele by crossing germline male mutants with C57BL/6 mice. (J:149241)

Strategy for generating the Lmx1b^{tm2(cre/ERT2)Rjo} and Lmx1b^{tm3(cre/ERT2)Rjo} alleles

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lmx1b (mouse) Summary of all recombinase alleles driven by Lmx1b. Alliance mouse Lmx1b gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lmx1b Mutation: 16 strains or lines available

Notes

Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells.

References

• Original: J:149241 Qiu Q, et al., Lmx1b-expressing cells in the mouse limb bud define a dorsal mesenchymal lineage compartment. Genesis. 2009 Apr;47(4):224-33
• All: 4 reference(s)