Kmt2a^tm1.1Brad
Targeted Allele Detail

Summary

• Symbol: Kmt2a^tm1.1Brad
• Name: lysine (K)-specific methyltransferase 2A; targeted mutation 1.1, Hugh JM Brady
• MGI ID: MGI:3849323
• Synonyms: Mll^-
• Gene: Kmt2a Location: Chr9:44714652-44792594 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Kmt2a^tm1.1Brad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149811
• Parent Cell Line: PC3 (ES Cell)
• Strain of Origin: 129S4/SvJae-Tg(Prm-cre)70Og

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was constructed where an upstream loxP site was cloned into a blunted NsiI site in intron 7 and a floxed neomycin resistance cassette was cloned into a BglII site in intron 9. Targeting took place in PC3 ES cells homozygous for the cre transgene Tg(Prm-cre)70Og, which expresses Cre recombinase in male germ cells. Cre mediated recombination subsequently removed exons 8 and 9 upon transmission from male chimeric mice. Offspring from these male chimeras were selected for further breeding. This is considered a null allele. (J:149811)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmt2a Mutation: 135 strains or lines available

References

• Original: J:149811 McMahon KA, et al., Mll has a critical role in fetal and adult hematopoietic stem cell self-renewal. Cell Stem Cell. 2007 Sep 13;1(3):338-45
• All: 2 reference(s)