Mecom^tm1Miku
Targeted Allele Detail

Summary

• Symbol: Mecom^tm1Miku
• Name: MDS1 and EVI1 complex locus; targeted mutation 1, Mineo Kurokawa
• MGI ID: MGI:3849413
• Synonyms: Evi-1^flox, Prdm3^flox
• Gene: Mecom Location: Chr3:30005445-30563937 bp, - strand Genetic Position: Chr3, 12.66 cM
• Alliance: Mecom^tm1Miku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149794
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 4 and a floxed neomycin cassette was placed downstream. After targeting and selection, transient expression of cre recombinase removed the neomycin cassette and left exon 4 floxed. Correct targeting was confirmed by Southern blotting. Expression of cre recombinase will remove exon 4, which encodes a zinc finger domain common to the three major protein isoforms of the gene locus. (J:149794)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mecom Mutation: 81 strains or lines available

References

• Original: J:149794 Goyama S, et al., Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells. Cell Stem Cell. 2008 Aug 7;3(2):207-20
• All: 8 reference(s)