Mecom^tm1.1Miku
Targeted Allele Detail

Summary

• Symbol: Mecom^tm1.1Miku
• Name: MDS1 and EVI1 complex locus; targeted mutation 1.1, Mineo Kurokawa
• MGI ID: MGI:3849414
• Synonyms: Evi-1^-
• Gene: Mecom Location: Chr3:30005445-30563937 bp, - strand Genetic Position: Chr3, 12.66 cM
• Alliance: Mecom^tm1.1Miku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149794
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 4 and a floxed neomycin cassette was placed downstream. After targeting and selection, transient expression of cre recombinase removed the neomycin cassette and exon 4. Exon 4 encodes a zinc finger domain common to the three major protein isoforms of the gene locus. Immunoblot analysis of homozygote embryonic fibroblasts indicated all three isoforms of the gene locus were absent. (J:149794)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mecom Mutation: 81 strains or lines available

References

• Original: J:149794 Goyama S, et al., Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells. Cell Stem Cell. 2008 Aug 7;3(2):207-20
• All: 8 reference(s)