Mfng^tm1Seco
Targeted Allele Detail

Summary

• Symbol: Mfng^tm1Seco
• Name: MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; targeted mutation 1, Susan E Cole
• MGI ID: MGI:3849429
• Synonyms: Mfng^delta1
• Gene: Mfng Location: Chr15:78640082-78657675 bp, - strand Genetic Position: Chr15, 37.69 cM
• Alliance: Mfng^tm1Seco page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149312
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An 898 bp fragment surrounding the first coding exon was replaced with a floxed neo cassette. Cre mediated recombination was then used to remove the floxed neo cassette. No phenotypic difference was reported between the neo in and neo out versions of this allele. Northern blot analysis detected the expression of an abnormal 2.0 kb RNA. RT-PCR and 5' RACE analysis indicate that this RNA includes exons 2-8 of the endogenous gene and a region from the first intron. Cell expression analysis indicates that the abnormal RNA would not produce a stable protein. (J:149312)

Expression

In Mice Carrying this Mutation:

23 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mfng Mutation: 35 strains or lines available

References

• Original: J:149312 Moran JL, et al., Manic fringe is not required for embryonic development, and fringe family members do not exhibit redundant functions in the axial skeleton, limb, or hindbrain. Dev Dyn. 2009 May 28;238(7):1803-1812
• All: 4 reference(s)