Plpp1^{Gt(RRD231)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Plpp1^{Gt(RRD231)Byg}
• Name: phospholipid phosphatase 1; gene trap RRD231, BayGenomics
• MGI ID: MGI:3849483
• Synonyms: Ppap2a^tr
• Gene: Plpp1 Location: Chr13:112937326-113004428 bp, + strand Genetic Position: Chr13, 63.93 cM, cytoband D2.2
• Alliance: Plpp1^{Gt(RRD231)Byg} page

Mutation origin

• Mutant Cell Line: RRD231
• Germline Transmission: Earliest citation of germline transmission: J:149782
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector pGT1Lxf was inserted into the first intron. Quantitative real time RT-PCR analysis detected very low levels of expression in all organs examined except the brain. Expression in the brain is not significantly different from littermate controls. Phosphatase activities are reduced in all tissue homogenates except the brain suggesting this is a hypomorphic allele. (J:149782)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRD231	CC199925 ; (MGI Seq Detail)	5' RACE	Chr13:112937356-112937798 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  27 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plpp1 Mutation: 35 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:149782 Tomsig JL, et al., Lipid phosphate phosphohydrolase type 1 (LPP1) degrades extracellular lysophosphatidic acid in vivo. Biochem J. 2009 May 1;419(3):611-8
• All: 3 reference(s)