Camk2a^tm1.1Oyam
Targeted Allele Detail

Summary

• Symbol: Camk2a^tm1.1Oyam
• Name: calcium/calmodulin-dependent protein kinase II alpha; targeted mutation 1.1, Yoko Yamagata
• MGI ID: MGI:3849851
• Synonyms: CaMKIIalpha(K42R)
• Gene: Camk2a Location: Chr18:61058704-61121224 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Camk2a^tm1.1Oyam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150046
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: Exon 2 was replaced with one in which an A to G transition results in an amino acid substitution of arginine for lysine at position 42 (K42R). This mutation abolishes the kinase activity of the protein product, as determined by enzymatic assays. A floxed neo cassette inserted downstream of exon 2 was removed by transient cre expression in ES cells. (J:150046)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Camk2a Mutation: 135 strains or lines available

References

• Original: J:150046 Yamagata Y, et al., Kinase-dead knock-in mouse reveals an essential role of kinase activity of Ca2+/calmodulin-dependent protein kinase IIalpha in dendritic spine enlargement, long-term potentiation, and learning. J Neurosci. 2009 Jun 10;29(23):7607-18
• All: 5 reference(s)