Tgfb1^tm2.1Doe
Targeted Allele Detail

Summary

• Symbol: Tgfb1^tm2.1Doe
• Name: transforming growth factor, beta 1; targeted mutation 2.1, Thomas Doetschman
• MGI ID: MGI:3849964
• Synonyms: Tgfb1^{flox ex6}
• Gene: Tgfb1 Location: Chr7:25386427-25404502 bp, + strand Genetic Position: Chr7, 13.98 cM
• Alliance: Tgfb1^tm2.1Doe page

Lung and salivary gland inflammation in Tgfb1^tm2.1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/? (b,e) and Tgfb1^tm1Doe/Tgfb1^tm2.1Doe Tg(Lck-cre)548Jxm/? (c,f) mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150024
• Parent Cell Line: KG-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Mutation details: A floxed neomycin cassette was placed upstream of exon 6 with an additional loxP site placed downstream of exon 6. The neomycin cassette was subsequently removed by transient expression of cre recombinase leaving exon 6 floxed. Germline transmission of the allele was confirmed by genomic PCR. (J:150024)

Generation of the Tgfb1^tm2.1Doe allele

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tgfb1 Mutation: 35 strains or lines available

References

• Original: J:150024 Azhar M, et al., Generation of mice with a conditional allele for transforming growth factor beta 1 gene. Genesis. 2009 Jun;47(6):423-31
• All: 31 reference(s)