Tg(Tbx18-icre)3Fech
Transgene Detail

Summary

• Symbol: Tg(Tbx18-icre)3Fech
• Name: transgene insertion 3, Feng Chen
• MGI ID: MGI:3850082
• Synonyms: Tbx18-Cre, Tbx18cre^Tg
• Transgene: Tg(Tbx18-icre)3Fech Location: unknown
• Alliance: Tg(Tbx18-icre)3Fech page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Tbx18-icre)3Fech expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tbx18 (MGI:1923615)

• Mutation details: A BAC clone (RP23-35307) carrying the entire mouse Tbx18 gene was recombineered such that the first exon was replaced with the coding sequence for codon-improved cre recombinase (icre) fused to a nuclear localization signial (NLS) and a Myc tag. An frt-flanked Amp(r) selection cassette followed the icre and was removed by transient Flp expression. The cre cassette carries a transcription termination signal so transcription from the Tbx18 promoter is terminated upstream of exon 2, effectively abolishing transcription of any Tbx18 sequence. The linearized construct was microinjected into fertilized oocytes from C57BL/6 x CBA hybrids. Three founders were obtained, crossed to R26R-LacZ reporter mice, and all showed similar reporter gene expression patterns, with line 2 having a lower level of cre activity. Lines 1 and 3 were used for further analysis. (J:150023)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tbx18 (mouse) Summary of all recombinase alleles driven by Tbx18. Comparative matrix view of recombinase activities Alliance mouse Tbx18 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:150023 Wang Y, et al., Cre/lox recombination in the lower urinary tract. Genesis. 2009 Jun;47(6):409-13
• All: 9 reference(s)