Tg(Il7-IL2RA)BRche
Transgene Detail

Summary

• Symbol: Tg(Il7-IL2RA)BRche
• Name: transgene insertion B, Ellen R Richie
• MGI ID: MGI:3850414
• Synonyms: IL-7.hCD25, IL7-hCD25
• Transgene: Tg(Il7-IL2RA)BRche Location: unknown
• Alliance: Tg(Il7-IL2RA)BRche page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Il7-IL2RA)BRche expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IL2RA (3559)

  Il2ra

  (MGI:96549)

• Mutation details: A BAC clone (RPCI-295A3) containing the murine Il7 locus and flanking sequences (96 kb 5'and 17 kb 3' of the Il7 locus) was modified by homologous recombination in E. coli to introduce the human IL2RA (CD25) cDNA and an FRT site-flanked Zeocin resistance gene at nucleotide position 1 of exon 1 in the Il7 mRNA, replacing the open reading frame of exon 1 entirely as well as the first nucleotide of Iintron 1. The recombinant BACs were transformed to the FLP-expressing E. coli strain FLP-294 to remove the Zeocin resistance gene. The linearized BAC was microinjected into pronuclei of fertilized FVB oocytes. 2 lines were established and line B (with 1 copy of the transgene) was characterized further. (J:149246)

Strategy for generating Tg(Il7-cre)CRche and Tg(Il7-IL2RA)BRche transgenic mice

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:149246 Repass JF, et al., IL7-hCD25 and IL7-Cre BAC transgenic mouse lines: new tools for analysis of IL-7 expressing cells. Genesis. 2009 Apr;47(4):281-7
• All: 2 reference(s)