Vcan^tm1.1Dzim
Targeted Allele Detail

Summary

• Symbol: Vcan^tm1.1Dzim
• Name: versican; targeted mutation 1.1, Dieter R Zimmermann
• MGI ID: MGI:3850559
• Synonyms: VCAN^(tm1Zim), VersicanV0/V2^-
• Gene: Vcan Location: Chr13:89803431-89890628 bp, - strand Genetic Position: Chr13, 45.5 cM
• Alliance: Vcan^tm1.1Dzim page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150124
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: An endoplasmic reticulum retention signal (KDEL) followed by a STOP codon and floxed neo cassette was inserted into exon 7 after codon E749. This exon encodes the GAG-alpha domain. Germ line, cre mediated recombination removed the neo cassette. Quantitative RT-PCR on brain extracts confirmed a 90% reduction in isoform V2 transcript expression and a decline of isoform V0 transcript to 20% (at P0) or 50% (at P30 and P100) of the levels in wild-type mice. Transcript expression of isoform V1 and V3 is unaffected. Immunohistochemistry confirmed the absence of protein expression of isoforms V0 and V2. (J:150124)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vcan Mutation: 164 strains or lines available

References

• Original: J:150124 Dours-Zimmermann MT, et al., Versican V2 assembles the extracellular matrix surrounding the nodes of ranvier in the CNS. J Neurosci. 2009 Jun 17;29(24):7731-42
• All: 5 reference(s)