Tg(Acta2-RAC1*G12V)33Pjgc
Transgene Detail

Summary

• Symbol: Tg(Acta2-RAC1*G12V)33Pjgc
• Name: transgene insertion 33, Pascal J Goldschmidt-Clermont
• MGI ID: MGI:3850849
• Synonyms: RacCA
• Transgene: Tg(Acta2-RAC1*G12V)33Pjgc Location: unknown
• Alliance: Tg(Acta2-RAC1*G12V)33Pjgc page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Acta2-RAC1*G12V)33Pjgc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RAC1 (5879)

  Rac1	(MGI:97845)

• Mutation details: The cDNA coding for human RAC1 was mutated with a glycine 12 to valine substitution. The cDNA of RacCA, including its polyadenylation tail, was cloned within the Smp-8 plasmid that contained a 3.6 kb segment of the 5 kb-region of SMA promoter. The SMA promoter was used to induce selective overexpression of RacCA in SMCs. The purified fragment was microinjectedinto fertilized mouse eggs from FVB/N females, (J:150479)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:150479 Hassanain HH, et al., Hypertension caused by transgenic overexpression of Rac1. Antioxid Redox Signal. 2007 Jan;9(1):91-100
• All: 4 reference(s)