Cd28^tm2.1Jmg
Targeted Allele Detail

Summary

• Symbol: Cd28^tm2.1Jmg
• Name: CD28 antigen; targeted mutation 2.1, Jonathan M Green
• MGI ID: MGI:3850854
• Synonyms: CD28-Y170F
• Gene: Cd28 Location: Chr1:60785547-60812521 bp, + strand Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
• Alliance: Cd28^tm2.1Jmg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:149888
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: A TAC to TTC mutation was created in exon 4 to substitute phenylalanine for tyrosine at position 170 (Y170F). A floxed neomycin cassette was inserted upstream of the exon and was subsequently removed by cre-mediated recombination. Expression of the mutant allele in splenocytes and thymocytes was similar to endogenous levels as determined by flow cytometry analysis. This mutation disrupts the motif responsible for interaction with the p85 subunit of PI3-kinase. (J:149888)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd28 Mutation: 52 strains or lines available

References

• Original: J:149888 Dodson LF, et al., Targeted knock-in mice expressing mutations of CD28 reveal an essential pathway for costimulation. Mol Cell Biol. 2009 Jul;29(13):3710-21
• All: 11 reference(s)