Cdc20^tm1.1Pzg
Targeted Allele Detail

Summary

• Symbol: Cdc20^tm1.1Pzg
• Name: cell division cycle 20; targeted mutation 1.1, Pumin Zhang
• MGI ID: MGI:3851133
• Synonyms: Cdc20^AAA
• Gene: Cdc20 Location: Chr4:118290098-118294540 bp, - strand Genetic Position: Chr4, 54.61 cM
• Alliance: Cdc20^tm1.1Pzg page

Aberrant mitoses in Cdc20^tm1.1Pzg/Cdc20^tm1.1Pzg MEFs

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150421
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

Allele Type: Targeted Mutations: Insertion, Nucleotide substitutions Mutation details: Two charged residues (K129 and R132) and one proline (P137) in the motif for Mad2 binding were changed to alanine and a floxed neo and kan resistance cassette was inserted in intron 3 via homologous recombination. The resistance cassette was then removed by Cre mediated recombination. (J:150421)

Generation of the Cdc20^tm1.1Pzg allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Cdc20^tm1.1Pzg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdc20 Mutation: 30 strains or lines available

References

• Original: J:150421 Li M, et al., Loss of spindle assembly checkpoint-mediated inhibition of Cdc20 promotes tumorigenesis in mice. J Cell Biol. 2009 Jun 15;185(6):983-94
• All: 3 reference(s)