Myrf^tm1Barr
Targeted Allele Detail

Summary

• Symbol: Myrf^tm1Barr
• Name: myelin regulatory factor; targeted mutation 1, Ben Barres
• MGI ID: MGI:3851143
• Synonyms: MRF^fl, Myrf^loxP
• Gene: Myrf Location: Chr19:10185636-10218112 bp, - strand Genetic Position: Chr19, 6.54 cM
• Alliance: Myrf^tm1Barr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150563
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was used to flank exon 8 of the endogenous gene with loxP sites. The vector also contained an FRT-flanked neomycin resistance cassette. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygous mice were crossed to the FlpER (129S4/SvJaeSor-Gt(ROSA)26Sor^tm1(FLP1)Dym) strain to remove the neo cassette, leaving a single FRT site upstream of exon 8 and the flanking loxP sites. (J:150563)

Expression

In Mice Carrying this Mutation:	55 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myrf Mutation: 101 strains or lines available

References

• Original: J:150563 Emery B, et al., Myelin gene regulatory factor is a critical transcriptional regulator required for CNS myelination. Cell. 2009 Jul 10;138(1):172-85
• All: 21 reference(s)