Tg(Tnnt2-rtTA,tetO-cre)1Wtp
Transgene Detail

Summary

• Symbol: Tg(Tnnt2-rtTA,tetO-cre)1Wtp
• Name: transgene insertion 1, William T Pu
• MGI ID: MGI:3851371
• Synonyms: ctTA-Cre, iTNT-cre, Tg(Tnnt2-rtTA,tetO-cre)t26Bz, TNT-icre
• Transgene: Tg(Tnnt2-rtTA,tetO-cre)1Wtp Location: unknown
• Alliance: Tg(Tnnt2-rtTA,tetO-cre)1Wtp page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Tnnt2-rtTA,tetO-cre)1Wtp expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Tnnt2 (24837)

  Tnnt2

  (MGI:104597)

• Mutation details: In one construct (Tnnt2-rtTA), a 0.5-kb rat Tnnt2 promoter was inserted in front of a nuclear-localized (nls) rtTA cDNA followed by an elongation factor-1 (EF1) polyadenylation signal. The Tnnt2-rtTA fragment was flanked by mouse H19 insulator sequences to prevent ectopic transcription influences from random insertion sites. The second construct (tetO-cre) was generated by cloning the nls-cre sequence downstream of the tetO-CMV minimal promoter fragment. Purified constructs were coinjected into fertilized FVB/N oocytes. Seven double-transgenic founders contained both constructs and transmitted them in Mendelian fashion indicating that both constructs were inserted at the same site. One line demonstrated reproducible recombination of the R26R reporter after doxycycline treatment and was propagated and maintained. (J:150452, J:157454)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Tnnt2 (rat) Summary of all recombinase alleles driven by Tnnt2. Alliance rat Tnnt2 gene page

Expression

In Mice Carrying this Mutation:

126 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:150452 Zhou B, et al., Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. J Clin Invest. 2009 Jun;119(6):1462-76
• All: 16 reference(s)