Tg(Myh6/tetO-PRKCA*)1Jmk
Transgene Detail

Summary

• Symbol: Tg(Myh6/tetO-PRKCA*)1Jmk
• Name: transgene insertion 1, Jeffery D Molkentin
• MGI ID: MGI:3851565
• Synonyms: dnPrkca, Tg(dnPrkca)
• Transgene: Tg(Myh6/tetO-PRKCA*)1Jmk Location: unknown
• Alliance: Tg(Myh6/tetO-PRKCA*)1Jmk page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(Myh6/tetO-PRKCA*)1Jmk expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  rabbit, European	PRKCA (100037720)

• Mutation details: The rabbit PRKCA cDNA carrying the dominant negative L368A mutation was fused to the cardiac-specific alpha myosin heavy chain (Myh6) mouse promoter. The promoter is minimally active/attenuated through the ablation of three GATA sites and two thyroid-like response elements. Seven copies of tetracycline-responsive promoter element (tetO) are fused to the promoter. (J:88510, J:132098)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:132098 Hambleton M, et al., Inducible and myocyte-specific inhibition of PKCalpha enhances cardiac contractility and protects against infarction-induced heart failure. Am J Physiol Heart Circ Physiol. 2007 Dec;293(6):H3768-71
• All: 2 reference(s)