Trp53^tm1.1Mcba
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1.1Mcba
• Name: transformation related protein 53; targeted mutation 1.1, Michelle Craig Barton
• MGI ID: MGI:3852473
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1.1Mcba page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150816
• Parent Cell Line: RJ2.2 (ES Cell)
• Strain of Origin: (C57BL/6Brd-Tyr^c-Brd x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin cassette was inserted into intron 10 and a TAP-tag cassette was inserted in-frame into exon 11 at the end of the coding sequence. The neomycin cassette was removed after targeting by transient expression of cre recombinase. The TAP epitope tag did not affect expression of function p53 protein produced as determined by numerous biochemical and phenotype analysis on homozygote mice. (J:150816)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:150816 Allton K, et al., Trim24 targets endogenous p53 for degradation. Proc Natl Acad Sci U S A. 2009 Jul 14;106(28):11612-6
• All: 1 reference(s)