Tg(Adora2a-cre)2MDkde
Transgene Detail

Summary

• Symbol: Tg(Adora2a-cre)2MDkde
• Name: transgene insertion 2M, Alban de Kerhcove d'Exaerde
• MGI ID: MGI:3852493
• Synonyms: A2a-cre, Adora2a-cre
• Transgene: Tg(Adora2a-cre)2MDkde Location: unknown
• Alliance: Tg(Adora2a-cre)2MDkde page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F1

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Adora2a-cre)2MDkde expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Adora2a (MGI:99402)

• Mutation details: A BAC clone of >160kb from a CJ7/129Sv Mouse BAC library (Invitrogen, formerly Genome Systems Inc.) containing the two exons of Adora2a, flanked by at least 70 kb of Adora2a 5' upstream sequences. This A2AR BAC was modified by homologous recombination in bacteria to replace the sequence of exon 1 (after the ATG transcription start site) with the cre recombinase coding sequence including 3 STOP codons. The purified BAC construct was injected into fertilized B6SJLF1 zygotes. Two founder lines, 2M and 9F, wer established. Mice were crossed to Rosa26-lacZ reporter mice to analyze cre-mediated recombination. Line 2M was used for further studies. (J:150475)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Adora2a (mouse) Summary of all recombinase alleles driven by Adora2a. Comparative matrix view of recombinase activities Alliance mouse Adora2a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:150475 Durieux PF, et al., D2R striatopallidal neurons inhibit both locomotor and drug reward processes. Nat Neurosci. 2009 Apr;12(4):393-5
• All: 25 reference(s)