Tcirg1^tm1.1Ywd
Targeted Allele Detail

Summary

• Symbol: Tcirg1^tm1.1Ywd
• Name: T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3; targeted mutation 1.1, Yoh Wada
• MGI ID: MGI:3852639
• Synonyms: a3-GFP, Tcirg1^floxGFP
• Gene: Tcirg1 Location: Chr19:3946050-3957133 bp, - strand Genetic Position: Chr19, 3.62 cM
• Alliance: Tcirg1^tm1.1Ywd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:150327
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: This allele contains a single Frt site and a single loxP site in intron 13 and the EGFP coding sequence, followed by another loxP site, inserted into the last exon. The modified gene encodes a fusion protein in which the C-terminal of TCIRG1 is joined to the N-terminal of EGFP by the linker sequence AALELVDPPGSIAT. EGFP fluorescence and the immunofluorescence signal for the a3 subunit of vacuolar-type ATPase (V-ATPase), which is encoded by the endogenous gene, are colocalized in the late endocytic compartments of mutant peritoneal macrophages. Cre recombinase excision of the loxP-flanked DNA segment generates a null allele. (J:150327)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tcirg1 Mutation: 36 strains or lines available

References

• Original: J:150327 Sun-Wada GH, et al., Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification. J Cell Sci. 2009 Jul 15;122(Pt 14):2504-13
• All: 4 reference(s)