Spin1^{Gt(RRZ449)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Spin1^{Gt(RRZ449)Byg}
• Name: spindlin 1; gene trap RRZ449, BayGenomics
• MGI ID: MGI:3852674
• Gene: Spin1 Location: Chr13:51254916-51306582 bp, + strand Genetic Position: Chr13, 26.04 cM
• Alliance: Spin1^{Gt(RRZ449)Byg} page

Mutation origin

• Mutant Cell Line: RRZ449
• Germline Transmission: Earliest citation of germline transmission: J:204704
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT2Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted between exon 3 and 4. Western blot analysis confirmed the absence of protein expression in E18.5 fetal gonads. (J:204704)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRZ449	CL791718 ; (MGI Seq Detail)	5' RACE	Chr13:51277288-51282008 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  185 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Spin1^{Gt(RRZ449)Byg} allele

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spin1 Mutation: 101 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)