Pikfyve^{Gt(AT0926)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Pikfyve^{Gt(AT0926)Wtsi}
• Name: phosphoinositide kinase, FYVE type zinc finger containing; gene trap AT0926, Wellcome Trust Sanger Institute
• MGI ID: MGI:3853063
• Synonyms: Pikfyve^beta-geo
• Gene: Pikfyve Location: Chr1:65225842-65317854 bp, + strand Genetic Position: Chr1, 32.94 cM, cytoband C3
• Alliance: Pikfyve^{Gt(AT0926)Wtsi} page

Spongiform degeneration in Pikfyve^{Gt(AT0926)Wtsi}/Pikfyve^{Gt(AT0926)Wtsi} neonatal hearts

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: AT0926
• Germline Transmission: Earliest citation of germline transmission: J:190368
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene trap vector inserted into intron 17. Quantitative real-time PCR detects both mutant and low levels of wild-type transcript in fibroblasts from homozygous mice. Western blot analysis detected low levels of protein in fibroblasts and multiple tissues from homozygous mice. The mutant protein fails to integrate into the Vac14 complex. (J:190368)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AT0926	CL866182 ; (MGI Seq Detail)	5' RACE	Chr1:65274214-65283694 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Schematic of the Pikfyve^{Gt(AT0926)Wtsi} allele

Expression

In Structures Affected by this Mutation:

22 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pikfyve Mutation: 116 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)