Prdm5^{Gt(AV0702)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Prdm5^{Gt(AV0702)Wtsi}
• Name: PR domain containing 5; gene trap AV0702, Wellcome Trust Sanger Institute
• MGI ID: MGI:3853086
• Synonyms: Prdm5^lacZ
• Gene: Prdm5 Location: Chr6:65755972-65913994 bp, + strand Genetic Position: Chr6, 30.32 cM, cytoband C1
• Alliance: Prdm5^{Gt(AV0702)Wtsi} page

Reduced bone formation in Prdm5^{Gt(AV0702)Wtsi}/Prdm5^{Gt(AV0702)Wtsi} mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: AV0702
• Germline Transmission: Earliest citation of germline transmission: J:185118
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene tarp cassette inserted in intron 2. Protein expression is reduced in homozygous embryonic fibroblasts to at most 10% that in wild-type cells. In adult organs from homozygous mice, except for brain, testis and lung, expression is reduced by at least 85 percent. Expression of the fusion reporter protein is seen in osteoblastic compartments. (J:185118)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AV0702	CL866216 ; (MGI Seq Detail)	5' RACE	Chr6:65756009-65771366 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  25 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Prdm5^{Gt(AV0702)Wtsi} allele

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prdm5 Mutation: 48 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)