Kpna6^{Gt(AJ0609)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Kpna6^{Gt(AJ0609)Wtsi}
• Name: karyopherin subunit alpha 6; gene trap AJ0609, Wellcome Trust Sanger Institute
• MGI ID: MGI:3856636
• Synonyms: Kpna6^-
• Gene: Kpna6 Location: Chr4:129537773-129566560 bp, - strand Genetic Position: Chr4, 63.28 cM, cytoband D2.3
• Alliance: Kpna6^{Gt(AJ0609)Wtsi} page

Mutation origin

• Mutant Cell Line: AJ0609
• Germline Transmission: Earliest citation of germline transmission: J:171441
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Modified isoform(s), Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: A gene trap cassette was inserted into intron 1. Western blot analysis confirmed the absence of protein expression in the brain, heart, lung, spleen and kidney of homozygous mice. Although protein is missing in most tissues examined, use of an alternative promoter and exon 1 (exon 1A) located downstream of the gene trap cassette leads to exclusive expression of a full-length and fully functional protein in the testis of homozygous mutant males. Sequencing of mRNA variants in testis revealed that only the shorter transcript starting from exon 1A is present at wild-type expression levels. (J:171441, J:311959)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AJ0609	CW989340 ; (MGI Seq Detail)	5' RACE	Chr4:129566469-129566548 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  17 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	37 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kpna6 Mutation: 44 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)