Ppm1b^{Gt(AW0406)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Ppm1b^{Gt(AW0406)Wtsi}
• Name: protein phosphatase 1B, magnesium dependent, beta isoform; gene trap AW0406, Wellcome Trust Sanger Institute
• MGI ID: MGI:3857220
• Synonyms: Ppm1b^d
• Gene: Ppm1b Location: Chr17:85264169-85331419 bp, + strand Genetic Position: Chr17, 55.13 cM, cytoband E4-E5
• Alliance: Ppm1b^{Gt(AW0406)Wtsi} page

Mutation origin

• Mutant Cell Line: AW0406
• Germline Transmission: Earliest citation of germline transmission: J:243815
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The lacZ and neomycin resistance genes containing gene trap vector inserted into intron 2 of the host gene. Transcrips from this allele will code for a fusion protein of the first 282 amino-acids of the host gene (which excludes the functional phosphatase domain), followed by beta-galactosidase and neo. Immunoblots show that a small amount of the wildtype L- and S-variants of the host gene peptides are still produced, possibly owing to splicing out of trap-containing intron 2. (J:243815)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AW0406	CW989972 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ppm1b Mutation: 38 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)