Mcm9^{Gt(AW0655)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Mcm9^{Gt(AW0655)Wtsi}
• Name: minichromosome maintenance 9 homologous recombination repair factor; gene trap AW0655, Wellcome Trust Sanger Institute
• MGI ID: MGI:3857270
• Synonyms: AW, AWO, Mcm9^AW0655
• Gene: Mcm9 Location: Chr10:53412411-53506535 bp, - strand Genetic Position: Chr10, 27.21 cM, cytoband B3
• Alliance: Mcm9^{Gt(AW0655)Wtsi} page

Mutation origin

• Mutant Cell Line: AW0655
• Germline Transmission: Earliest citation of germline transmission: J:177420
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: The gene trap vector inserted in intron 3. RT-PCR analysis detected reduced levels of the long and short isoforms. Expression of Asf1a is about similar to wild-type levels. (J:177420)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AW0655	CW990027 ; (MGI Seq Detail)	5' RACE	Chr10:53498851-53499006 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  26 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Schematic of the insertion sites of the Asf1a^{Gt(AL0673)Wtsi}, Del(10Mcm9-Asf1a)1Byg, Mcm9^{Gt(AW0655)Wtsi}, and Mcm9^Gt(XG743)Byg gene traps

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Mcm9^{Gt(AW0655)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm9 Mutation: 75 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)