Mff^{Gt(AZ0438)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Mff^{Gt(AZ0438)Wtsi}
• Name: mitochondrial fission factor; gene trap AZ0438, Wellcome Trust Sanger Institute
• MGI ID: MGI:3857630
• Synonyms: Mff^gt
• Gene: Mff Location: Chr1:82702611-82730115 bp, + strand Genetic Position: Chr1, 42.45 cM
• Alliance: Mff^{Gt(AZ0438)Wtsi} page

Mutation origin

• Mutant Cell Line: AZ0438
• Germline Transmission: Earliest citation of germline transmission: J:200469
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: Gene trap vector pGT01xr, containing a splice acceptor and a lacZ/neomycin phosphotransferase (betageo) fusion gene, was inserted immediately after the sequence 5'-GCACTCCTCTGTCTGCCTTG-3' in intron 4. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts (MEFs). (J:230736)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AZ0438	CW990405 ; (MGI Seq Detail)	5' RACE	Chr1:82706995-82709002 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  6 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mff Mutation: 25 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)