Nono^{Gt(YHA266)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Nono^{Gt(YHA266)Byg}
• Name: non-POU-domain-containing, octamer binding protein; gene trap YHA266, BayGenomics
• MGI ID: MGI:3866756
• Gene: Nono Location: ChrX:100473295-100492193 bp, + strand Genetic Position: ChrX, 44.14 cM
• Alliance: Nono^{Gt(YHA266)Byg} page

Mutation origin

• Mutant Cell Line: YHA266
• Germline Transmission: Earliest citation of germline transmission: J:189236
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: The gene trap vector inserted in intron 2. PCR analysis detected the presence of a truncated transcript containing the first 2 exons and the gene trap cassette in fibroblasts. Immunohistochemical analysis confirmed the absence of protein expression in tissues from hemizygous mice. (J:189236)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  YHA266	CZ404241 ; (MGI Seq Detail)	5' RACE	ChrX:100473294-100474155 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  103 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nono Mutation: 30 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 9 reference(s)