Wwp2^{Gt(HMA140)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Wwp2^{Gt(HMA140)Byg}
• Name: WW domain containing E3 ubiquitin protein ligase 2; gene trap HMA140, BayGenomics
• MGI ID: MGI:3868073
• Synonyms: miR-140^GT, Wwp2^Gt
• Gene: Wwp2 Location: Chr8:108162997-108285227 bp, + strand Genetic Position: Chr8, 54.15 cM
• Alliance: Wwp2^{Gt(HMA140)Byg} page

Mutation origin

• Mutant Cell Line: HMA140
• Germline Transmission: Earliest citation of germline transmission: J:228709
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The lacZ-containing gene trap vector inserted between exon 3 and 4. Western blot analysis confirmed the absence of protein expression. (J:228709)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  HMA140	CZ595187 ; (MGI Seq Detail)	5' RACE	Chr8:108163036-108179696 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  368 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wwp2 Mutation: 373 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

Craniofacial phenotypes may be associated with reduced Mir140 expression rather than reduced Wwp2 expression. J:278573

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 7 reference(s)