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Letm1Gt(CC0204)Wtsi
Gene trapped Allele Detail
Summary
Symbol: Letm1Gt(CC0204)Wtsi
Name: leucine zipper-EF-hand containing transmembrane protein 1; gene trap CC0204, Wellcome Trust Sanger Institute
MGI ID: MGI:3870303
Synonyms: Letm1-
Gene: Letm1  Location: Chr5:33897017-33940061 bp, - strand  Genetic Position: Chr5, 17.83 cM
Alliance: Letm1Gt(CC0204)Wtsi page
Small size and decreased cell proliferation in Letm1Gt(DD0926)Wtsi/Letm1+ and Letm1Gt(CC0204)Wtsi/Letm1+ mouse embryos

Show the 1 phenotype image(s) involving this allele.

Mutation
origin
Mutant Cell Line:  CC0204
Germline Transmission:  Earliest citation of germline transmission: J:222017
Parent Cell Line:  E14TG2a.4 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Sanger Inst. Gene Trap Res.
Mutation
description
Allele Type:    Gene trapped (Null/knockout)
Mutation:    Insertion of gene trap vector     Vector: pGT0lxfT2
 
Mutation detailsThe exogenous gene-trap vector sequence, containing an in-frame splice acceptor site followed by coding sequence for a beta-galactosidase/neomycin (beta-Geo) fusion protein, was inserted into the intron following exon 3 (CC0204). The insertion of a trapping vector after exon 3 creates fusion transcripts and premature stop codons, thus preventing the synthesis of full-length mRNA. Full-length mRNA levels are reduced by 50% in heterozygous mutant mice. (J:222017)
Sequence Tags: 
Sequence tag details (1 tag)
Genome Context: 
Genome Browser view of this mutation
Generation of the Letm1Gt(DD0926)Wtsi and Letm1Gt(CC0204)Wtsi alleles
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 5 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Letm1 Mutation:  27 strains or lines available
References
Original:  J:222017 Jiang D, et al., Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome. Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):E2249-54
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory