Letm1Gt(CC0204)Wtsi
Gene trapped Allele Detail
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Symbol: |
Letm1Gt(CC0204)Wtsi |
Name: |
leucine zipper-EF-hand containing transmembrane protein 1; gene trap CC0204, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:3870303 |
Synonyms: |
Letm1- |
Gene: |
Letm1 Location: Chr5:33897017-33940061 bp, - strand Genetic Position: Chr5, 17.83 cM
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Alliance: |
Letm1Gt(CC0204)Wtsi page
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Small size and decreased cell proliferation in Letm1Gt(DD0926)Wtsi/Letm1+ and Letm1Gt(CC0204)Wtsi/Letm1+ mouse embryos
Show the 1 phenotype image(s) involving this allele.
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Mutant Cell Line: |
CC0204 |
Germline Transmission: |
Earliest citation of germline transmission:
J:222017
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Parent Cell Line: |
E14TG2a.4 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Project Collection: |
Sanger Inst. Gene Trap Res.
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Allele Type: |
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Gene trapped (Null/knockout) |
Mutation: |
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Insertion of gene trap vector
Vector: pGT0lxfT2
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Mutation details: The exogenous gene-trap vector sequence, containing an in-frame splice acceptor site followed by coding sequence for a beta-galactosidase/neomycin (beta-Geo) fusion protein, was inserted into the intron following exon 3 (CC0204). The insertion of a trapping vector after exon 3 creates fusion transcripts and premature stop codons, thus preventing the synthesis of full-length mRNA. Full-length mRNA levels are reduced by 50% in heterozygous mutant mice.
(J:222017)
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Sequence Tags: |
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Sequence tag details (1 tag)
Tag ID |
GenBank ID |
Method |
Tag Location (trapped strand)* |
Select |
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Genome Context: |
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Genome Browser view of this mutation
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Generation of the Letm1Gt(DD0926)Wtsi and Letm1Gt(CC0204)Wtsi alleles |
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Letm1 Mutation: |
27 strains or lines available
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Original: |
J:222017 Jiang D, et al., Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome. Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):E2249-54 |
All: |
1 reference(s) |
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