Casz1^{Gt(CJ0565)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Casz1^{Gt(CJ0565)Wtsi}
• Name: castor zinc finger 1; gene trap CJ0565, Wellcome Trust Sanger Institute
• MGI ID: MGI:3870873
• Synonyms: Casz1^betageo
• Gene: Casz1 Location: Chr4:148888886-149039346 bp, + strand Genetic Position: Chr4, 78.87 cM
• Alliance: Casz1^{Gt(CJ0565)Wtsi} page

Edema, heart failure, and heart abnormalities in E16.5 Casz1^{Gt(CJ0565)Wtsi}/Casz1^{Gt(CJ0565)Wtsi} fetuses

Show the 7 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: CJ0565
• Germline Transmission: Earliest citation of germline transmission: J:242127
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxrT2
• Mutation details: A gene trap containing a beta-geo reporter inserted into the sequences after exon 9, resulting in a truncated protein in which 10 of the 11 zinc fingers are lost. Real time PCR analysis revealed no detectable mRNA in the heart of E12.5 homozygous mutant embryos. Western blot analysis confirmed the absence of protein expression of both the full-length (Casz1a) and shorter (Casz1b) isoform in E14.5 whole heart lysates. (J:242127)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  CJ0565	DU636079 ; (MGI Seq Detail)	5' RACE	multiple
  * GRCm39; (strand of gene trap mutagenesis)

Generation of the Casz1^{Gt(CJ0565)Wtsi} allele

Disease models

Expression

In Mice Carrying this Mutation:	68 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casz1 Mutation: 345 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 2 reference(s)