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Lemd2Gt(DD0639)Wtsi
Gene trapped Allele Detail
Summary
Symbol: Lemd2Gt(DD0639)Wtsi
Name: LEM domain containing 2; gene trap DD0639, Wellcome Trust Sanger Institute
MGI ID: MGI:3871218
Synonyms: Lemd2gt
Gene: Lemd2  Location: Chr17:27408574-27423443 bp, - strand  Genetic Position: Chr17, 13.82 cM
Alliance: Lemd2Gt(DD0639)Wtsi page
Mutation
origin
Mutant Cell Line:  DD0639
Germline Transmission:  Earliest citation of germline transmission: J:221495
Parent Cell Line:  E14TG2a.4 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Project Collection: Sanger Inst. Gene Trap Res.
Mutation
description
Allele Type:    Gene trapped (Null/knockout, Reporter)
Mutation:    Insertion of gene trap vector     Vector: pGT1lxrT2
 
Mutation detailsThe gene trap introduced a sequence between exons 3 and 4 of the gene that contains a splice acceptor site (SA), a cDNA encoding the beta-galactosidase reporter linked to neomycin (geo), and a SV40 cleavage/polyadenylation site (pA). While the mutant allele is predicted to generate an ~175-kDa fusion protein, a full length expression product was not detected. Smaller presumptive degredation products were seen on Western blot, albeit at reduced levels. (J:221495)
Sequence Tags: 
Sequence tag details (1 tag)
Genome Context: 
Genome Browser view of this mutation
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 93 assay results
In Structures Affected by this Mutation: 11 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Lemd2 Mutation:  20 strains or lines available
References
Original:  J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory