Letm1^{Gt(DD0926)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Letm1^{Gt(DD0926)Wtsi}
• Name: leucine zipper-EF-hand containing transmembrane protein 1; gene trap DD0926, Wellcome Trust Sanger Institute
• MGI ID: MGI:3871268
• Synonyms: Letm1^-
• Gene: Letm1 Location: Chr5:33897017-33940061 bp, - strand Genetic Position: Chr5, 17.83 cM
• Alliance: Letm1^{Gt(DD0926)Wtsi} page

Small size and decreased cell proliferation in Letm1^{Gt(DD0926)Wtsi}/Letm1⁺ and Letm1^{Gt(CC0204)Wtsi}/Letm1⁺ mouse embryos

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: DD0926
• Germline Transmission: Earliest citation of germline transmission: J:222017
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT1lxrT2
• Mutation details: The exogenous gene-trap vector sequence, containing an in-frame splice acceptor site followed by coding sequence for a beta-galactosidase/neomycin (beta-Geo) fusion protein, was inserted into the intron following exon 1 (DD0926). The insertion of a trapping vector after exon 1 creates fusion transcripts and premature stop codons, thus preventing the synthesis of full-length mRNA. Full-length mRNA levels are reduced by 50% in heterozygous mutant mice. (J:222017)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  DD0926	DU636475 ; (MGI Seq Detail)	5' RACE	Chr5:33939817-33939988 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  3 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Letm1^{Gt(DD0926)Wtsi} and Letm1^{Gt(CC0204)Wtsi} alleles

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Letm1 Mutation: 27 strains or lines available

References

• Original: J:222017 Jiang D, et al., Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome. Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):E2249-54
• All: 1 reference(s)