Arhgap42^{Gt(CE0477)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Arhgap42^{Gt(CE0477)Wtsi}
• Name: Rho GTPase activating protein 42; gene trap CE0477, Wellcome Trust Sanger Institute
• MGI ID: MGI:3871380
• Synonyms: GRAF3^gt
• Gene: Arhgap42 Location: Chr9:8994330-9239106 bp, - strand Genetic Position: Chr9, 2.46 cM, cytoband A1
• Alliance: Arhgap42^{Gt(CE0477)Wtsi} page

Mutation origin

• Mutant Cell Line: CE0477
• Germline Transmission: Earliest citation of germline transmission: J:221301
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxfT2v
• Mutation details: 5' RACE revealed the gene trap cassette (beta-geo) is located in intron 3. Expressed mRNA was strongly but not completely depleted in all tissues evaluated indicating that this mutation results in a hypomorphic allele. Inclusion of the LacZ/neo insertion cassette into the aberrantly spliced transcript results in a heterologous protein that contains GRAF3 amino acids 1-104 fused in frame to the beta-Gal reporter gene. As expected, beta-Gal staining in gene trap mice closely mimicked endogenous gene expression with strong and selective staining within the heart, mesenteric, skeletal muscle and brain arterioles and the SMC layers of the GI tract. (J:221301)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  CE0477	DU636587 ; (MGI Seq Detail)	5' RACE	Chr9:9155303-9238924 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arhgap42 Mutation: 53 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)