Trim71^{Gt(H004C06)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Trim71^{Gt(H004C06)Wrst}
• Name: tripartite motif-containing 71; gene trap H004C06, German Gene Trap Consortium
• MGI ID: MGI:3875666
• Synonyms: Lin41^gt, Lin41^{Gt(lacZneo)fgw}
• Gene: Trim71 Location: Chr9:114340336-114393437 bp, - strand Genetic Position: Chr9, 64.54 cM
• Alliance: Trim71^{Gt(H004C06)Wrst} page

Mutation origin

• Mutant Cell Line: H004C06
• Germline Transmission: Earliest citation of germline transmission: J:226960
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: FlipROSAbgeo0
• Mutation details: The gene trap vector FlipROSAbgeo0 vector inserted within the second intron at position 7957 of the gene. Western blot analysis on extracts from E9.5 embryos confirmed the loss of wild type encoded protein in homozygous mutant mice. A chimeric protein with beta-galactosidase activity is expressed from this allele. (J:226960)
• Sequence Tags: Sequence tag details (3 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  3SH004C06	ET637178 ; (MGI Seq Detail)	3'Splinkerette PCR	Chr9:114345220-114346055 bp (-)
  H004C06	DU823856 ; (MGI Seq Detail)	5' RACE	Chr9:114354029-114354111 bp (-)
  5SH004C06	ET637179 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr9:114346073-114346315 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  187 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trim71 Mutation: 170 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)