Arfgef1^{Gt(CSH465)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Arfgef1^{Gt(CSH465)Byg}
• Name: ARF guanine nucleotide exchange factor 1; gene trap CSH465, BayGenomics
• MGI ID: MGI:3878593
• Synonyms: Arfgef1^geo, BIG1-deficient
• Gene: Arfgef1 Location: Chr1:10207796-10302895 bp, - strand Genetic Position: Chr1, 2.36 cM
• Alliance: Arfgef1^{Gt(CSH465)Byg} page

Mutation origin

• Mutant Cell Line: CSH465
• Germline Transmission: Earliest citation of germline transmission: J:248338
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap cassette was inserted between exon 35 and exon 36, corresponding to sequences encoding the C-terminus of the protein. The encoded protein is undetectable in homozygous mutant brains using antibody against an N-terminal epitope or a C-terminal epitope, confirming the complete absence of the encoded protein for this allele. (J:248338)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  CSH465	DX934856 ; (MGI Seq Detail)	5' RACE	Chr1:10212803-10217758 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  7 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	64 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Arfgef1 Mutation: 104 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)