Uso1^{Gt(YTA025)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Uso1^{Gt(YTA025)Byg}
• Name: USO1 vesicle docking factor; gene trap YTA025, BayGenomics
• MGI ID: MGI:3880692
• Gene: Uso1 Location: Chr5:92285797-92350657 bp, + strand Genetic Position: Chr5, 46.05 cM, cytoband E3
• Alliance: Uso1^{Gt(YTA025)Byg} page

Dispersed Golgi architecture in Uso1^{Gt(YTA025)Byg}/Uso1^{Gt(YTA025)Byg} and Uso1^{Gt(AW0562)Wtsi}/Uso1^{Gt(AW0562)Wtsi} blastocysts

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: YTA025
• Germline Transmission: Earliest citation of germline transmission: J:195576
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: The gene trap vector inserted 340 bp downstream of exon 12. Immunohistochemistry confirmed the lack of protein expression in blastocysts. (J:195576)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  YTA025	EI183104 ; (MGI Seq Detail)	5' RACE	Chr5:92318621-92329351 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  21 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Generation of the Uso1^{Gt(YTA025)Byg} and Uso1^{Gt(AW0562)Wtsi} alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Uso1 Mutation: 63 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)