Dst^{Gt(E182H05)Wrst}
Gene trapped Allele Detail

Summary

• Symbol: Dst^{Gt(E182H05)Wrst}
• Name: dystonin; gene trap E182H05, German Gene Trap Consortium
• MGI ID: MGI:3917429
• Synonyms: Dst^Gt
• Gene: Dst Location: Chr1:33947306-34347742 bp, + strand Genetic Position: Chr1, 12.91 cM
• Alliance: Dst^{Gt(E182H05)Wrst} page

Mutation origin

• Mutant Cell Line: E182H05
• Germline Transmission: Earliest citation of germline transmission: J:228781
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Modified isoform(s))
• Mutation: Insertion of gene trap vector Vector: rsFROSAbgeo0s
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The gene trap inserted within the coding region of ABD CH, disrupting the Dst 1 and 2 actin-binding domain-containing isoforms transcribed from upstream promoters and generating a Dystonin-lacZ fusion product. RT-PCR analysis indicates that these isoforms are either absent or barely detectable in homozygotes. In addition, the F1Ex system relies on the sequential use of the Cre and FLP recombinases for the conditional inactivation and reactivation of the gene trap alleles. The first site-specific recombination using FLP recombinase reverses the gene trap cassette from the original mutagenic orientation to a non-mutagenic, inverted orientation. Subsequent recombination using cre recombinase leads to inversion of the transgene back to the mutagenic direct orientation. (J:228781)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  5SE182H05	FI572887 ; (MGI Seq Detail)	5'Splinkerette PCR	Chr1:34141649-34142166 bp (+)
  3SE182H05	FI572886 ; (MGI Seq Detail)	3'Splinkerette PCR	unknown
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  190 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dst Mutation: 557 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 9 reference(s)