Mbd5^{Gt(Ayu21-B205)Imeg}
Gene trapped Allele Detail

Summary

• Symbol: Mbd5^{Gt(Ayu21-B205)Imeg}
• Name: methyl-CpG binding domain protein 5; gene trap Ayu21-B205, Institute of Molecular Embryology and Genetics
• MGI ID: MGI:3923358
• Synonyms: Mbd5^{Gt(pU-21B)205Imeg}
• Gene: Mbd5 Location: Chr2:48839511-49209702 bp, + strand Genetic Position: Chr2, 28.43 cM, cytoband C1
• Alliance: Mbd5^{Gt(Ayu21-B205)Imeg} page

Mutation origin

• Mutant Cell Line: Ayu21-B205
• Germline Transmission: Earliest citation of germline transmission: J:212073
• Parent Cell Line: KTPU8 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Gene trapped (Hypomorph)
• Mutation: Insertion of gene trap vector Vector: pU-21B
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. Gene-trap construct pU-21B was randomly inserted in intron 2. The construct pU-21B consists of 1.8 kb of an intron and a splice acceptor sequence from the mouse En-2 gene, a promoterless beta-geo gene containing three stop codons in-frame with the ATG, and a polyadenylation signal. (J:212073)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  Ayu21-B205	AB257593 ; (MGI Seq Detail)	5' RACE	Chr2:48908271-48908336 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  13 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	38 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbd5 Mutation: 68 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 6 reference(s)