Prss8^{Gt(IST10122F12)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Prss8^{Gt(IST10122F12)Tigm}
• Name: serine protease 8 (prostasin); gene trap IST10122F12, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:3930527
• Gene: Prss8 Location: Chr7:127524889-127529266 bp, - strand Genetic Position: Chr7, 69.84 cM
• Alliance: Prss8^{Gt(IST10122F12)Tigm} page

Mutation origin

• Mutant Cell Line: IST10122F12
• Germline Transmission: Earliest citation of germline transmission: J:188121
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. The retroviral gene trap vector inserted into intron 2. This insertion results in a null mutation by placing a strong splice acceptor site in intron 2, leading to formation of a truncated mRNA fused to a beta-geo reporter gene. RT-PCR confirmed absence of mRNA in the skin, intestine, kidneys and lungs and Western blot analysis confirmed absence of protein. (J:214122)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST10122F12BBF1	EF719679 ; (MGI Seq Detail)	inverse PCR	Chr7:127528395-127528952 bp (+)
  IST10122F12HMF1	EF722928 ; (MGI Seq Detail)	inverse PCR	Chr7:127528395-127528900 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  4 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	9 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Prss8 Mutation: 24 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)