Cfap54^{Gt(IST10309B2)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Cfap54^{Gt(IST10309B2)Tigm}
• Name: cilia and flagella associated protein 54; gene trap IST10309B2, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:3942561
• Synonyms: Cfap54^gt
• Gene: Cfap54 Location: Chr10:92611481-92917480 bp, - strand Genetic Position: Chr10, 47.44 cM
• Alliance: Cfap54^{Gt(IST10309B2)Tigm} page

Mutation origin

• Mutant Cell Line: IST10309B2 More than one mutation may be present.
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: The gene-trapping cassette inserts into the first intron of the gene and is expected to result in truncation of the transcript after exon 1. qRT-PCR analysis revealed substantial transcript expression in wild-type testis but only a very low level of transcript in homozygous mutant testis. (J:228444)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST10309B2BBF1	EF735345 ; (MGI Seq Detail)	inverse PCR	Chr9:40536790-40537337 bp (-)
  IST10309B2HMF2	EF735346 ; (MGI Seq Detail)	inverse PCR	Chr10:92907187-92907349 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfap54 Mutation: 73 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Primary Ciliary Dyskinesia in homozygous mice (J:228444).

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)