Chchd4^{Gt(IST11943B12)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Chchd4^{Gt(IST11943B12)Tigm}
• Name: coiled-coil-helix-coiled-coil-helix domain containing 4; gene trap IST11943B12, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:4038368
• Synonyms: Chchd4^-
• Gene: Chchd4 Location: Chr6:91441258-91450405 bp, - strand Genetic Position: Chr6, 40.53 cM
• Alliance: Chchd4^{Gt(IST11943B12)Tigm} page

Mutation origin

• Mutant Cell Line: IST11943B12
• Germline Transmission: Earliest citation of germline transmission: J:250035
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: Trapped gene association determined by genome coordinate overlap and strand. Immunoblot analysis confirmed the absence of protein expression in extracts from homozygous mutant embryos staged at E5.5-E6.0. Adult heterozygotes showed a ~50% reduction of CHCHD4 protein levels in various organs with no detectable changes in the abundance of AIFM1 protein. (J:250035, J:256078)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST11943B12BBF1	EF867259 ; (MGI Seq Detail)	inverse PCR	Chr6:91444773-91445267 bp (+)
  IST11943B12HMF1	EF866218 ; (MGI Seq Detail)	inverse PCR	Chr6:91444773-91444931 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  26 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

8 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Chchd4 Mutation: 25 strains or lines available

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 4 reference(s)