Azin2^{Gt(IST12418H6)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Azin2^{Gt(IST12418H6)Tigm}
• Name: antizyme inhibitor 2; gene trap IST12418H6, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:4075460
• Synonyms: Adc^{Gt(IST12418H6)Tigm}, Azin2^betaGeo
• Gene: Azin2 Location: Chr4:128824026-128856235 bp, - strand Genetic Position: Chr4, 62.48 cM
• Alliance: Azin2^{Gt(IST12418H6)Tigm} page

Mutation origin

• Mutant Cell Line: IST12418H6 More than one mutation may be present.
• Germline Transmission: Earliest citation of germline transmission: J:204392
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: The gene trap vector inserted between exon 4 and 5. RT-PCR confirmed the absence of transcript expression in testis, brain, adrenal gland and pancreas. (J:204392)
• Sequence Tags: Sequence tag details (3 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST12418H6BBF1	EF920809 ; (MGI Seq Detail)	inverse PCR	Chr14:34434687-34434895 bp (+)
  IST12418H6HMF1	EF929325 ; (MGI Seq Detail)	inverse PCR	Chr4:128852183-128852442 bp (+)
  IST12418H6BBF1	JM197500 ; (MGI Seq Detail)	inverse PCR	Chr14:34434687-34434895 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

Generation of the Azin2^{Gt(IST12418H6)Tigm} allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Azin2 Mutation: 19 strains or lines available

Notes

The mutant cell line was initially identified as a 'mixed line' (characterized by multiple sequence tags for the same cell line that align to different locations in the genome). However, subsequent expansion of sequencing clone TIGM was unable to confirm the Chr 14 map location.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 5 reference(s)