Amfr^{Gt(IST12797A11)Tigm}
Gene trapped Allele Detail

Summary

• Symbol: Amfr^{Gt(IST12797A11)Tigm}
• Name: autocrine motility factor receptor; gene trap IST12797A11, Texas A&M Institute for Genomic Medicine
• MGI ID: MGI:4093875
• Synonyms: gp78-KO
• Gene: Amfr Location: Chr8:94698216-94739301 bp, - strand Genetic Position: Chr8, 45.96 cM
• Alliance: Amfr^{Gt(IST12797A11)Tigm} page

Mutation origin

• Mutant Cell Line: IST12797A11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: Lex3.13 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: VICTR76 Vector Type: gene trap
• Mutation details: Sequence tags for this gene trap insertion do not overlap a single known gene in the trapped gene orientation. It is possible that sequence tags for this gene trap overlap multiple genes in the trapped gene orientation, one or more genes in reverse orientation (not disrupted by the gene trap mechanism), or no known genes (using current gene model coordinates). J:221247 reports that the gene-trapping vector was inserted in the first intron downstream of the ATG codon between the first and second exons, which block production of endogenous transcription. RT-PCR and Western blot analysis confirmed absence of mRNA or protein in homozygous embryonic lysate or liver tissue. (J:221247)
• Sequence Tags: Sequence tag details (2 tags)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  IST12797A11HMF1	EF975687 ; (MGI Seq Detail)	inverse PCR	Chr8:94738100-94738301 bp (+)
  IST12797A11BBF1	EF946228 ; (MGI Seq Detail)	inverse PCR	Chr8:94738098-94738151 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  2 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Amfr Mutation: 42 strains or lines available

Notes

Phenotypic Similarity to Human Syndrome: Nonalcoholic Steatohepatitis (NASH) J:221247.

References

• Original: J:141210 Mouse Genome Informatics (MGI) and National Center for Biotechnology Information (NCBI), Mouse Gene Trap Data Load from dbGSS. Database Download. 2008
• All: 3 reference(s)